Chromatography

Chromatography is a method used to separate and identify the different parts of a mixture. It involves a stationary phase (like a solid or liquid) and a mobile phase (like a liquid or gas). As the mixture moves with the mobile phase, its components travel at different speeds, allowing them to be separated and studied.

Thin Layer Chromatography

Thin Layer Chromatography (TLC) is a quick and simple way to separate and identify components in a mixture. A small spot of the mixture is placed on a coated plate, and as a liquid solvent moves up the plate, it carries the components with it. Different parts move at different speeds (depending on their interaction with the plate), creating distinct spots. By comparing the spots to known samples, scientists can identify and analyze the mixture. It's commonly used to monitor reactions or detect compounds in substances.

Column Chromatography

Column chromatography separates components of a mixture by passing it through a column filled with a solid material (stationary phase). As a liquid flows through, the components move at different speeds depending on how strongly they interact with the stationary phase. This separates the mixture into distinct parts, which can then be collected for further analysis. It’s commonly used to purify chemicals or isolate specific compounds

Gaz Chromatography

Gas Chromatography (GC) separates and analyzes compounds that can be vaporized. A sample is injected into the system, turned into gas, and carried by an inert gas (mobile phase) through a column coated with a stationary phase. Different compounds move through the column at different speeds based on their properties, like boiling points or polarity. As they exit, a detector identifies them. GC is widely used to analyze volatile compounds in perfumes, fuels, or environmental samples.

High Performance Liquid Chromatography

High-Performance Liquid Chromatography (HPLC) is a powerful technique to separate, identify, and quantify compounds in a mixture. A liquid sample is injected into a column packed with tiny particles (stationary phase) while a solvent (mobile phase) pushes it through. Components interact differently with the stationary phase, so they come out at different times. Detectors measure them as they exit. HPLC is often used in pharmaceuticals, food testing, and environmental analysis for precise and fast results

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